detection of bluetongue virus in aborted lamb fetuses in chaharmahal va bakhtiari and isfahan provinces, by rt-pcr method

to consider of seroprevalence and to determine the focus of brucellosis propagation in domestic animal population of different regions of mazandaran province, within 5 years (2006- 2010), blood samples of 4845 numbers of sheep and goats of different districts of the province were taken. due to continental situations, aggregation and dispersion of domestic animal population of the province, we gathered information from ranchers who had come to veterinary networks of different districts of the province to diagnosis, treatment and the get the compensation for accession and complication of the disease. we also took blood samples from all sick animals and we did random sampling for other livestock existing around contamination focus point. after serum separation, rose bengal test was done for all samples as the primary recognizing test, and then right and 2-mercaptoethanole test were done on positive serums to make the final diagnosis. the results obtained from this research showed that 457 of all tested serum samples (9.44 %) were positive in supplementary test. among all regions of the province, sari district with 15.35 % of contamination, allocates the most amounts of reactor livestock to itself, and is the main focus point of disease outbreak in the province. districts such as noor, nowshahr- chaloos and babol with 14.09, 13.02 and 12.01 percent are in next ranks of contamination, respectively. also, at this time, juibar district had the least contamination (0.84 %). it seems that ranchers raise awareness and prevent the import and impermissible slaughter of contaminated animals, vaccination for all domestic animals and studies its portion effect in vaccinated animals, have an important role in control and prevention of brucellosis disease in domestic animal population of mazandaran province.