screening for starch-hydrolysing bacteria

SCREENING FOR STARCH-HYDROLYSING BACTERIA

Screening of3000 soil samples collected from cities of four different provinces of Iran for starch-hydrolysing bacteria revealed that the nature is enriched with Streptomyces species capable of producing amylolytic enzymes. Among the bacterial isolates, one of the high starch-degrading strains was selected for further microbiological identification and also amylolytic enzyme(s) characteriza...

A starch-accumulating mutant of Arabidopsis thaliana deficient in a chloroplastic starch-hydrolysing enzyme.

The aim of this work was to identify enzymes that participate in the degradation of transitory starch in Arabidopsis. A mutant line was isolated by screening leaves at the end of the night for the presence of starch. The mutant had a higher starch content than the wild-type throughout the diurnal cycle. This accumulation was due to a reduction in starch breakdown, leading to an imbalance betwee...

SCREENING FOR ANTIBIOTIC RESISTANT BACTERIA IN URINE SAMPLES

Chronic urinary tract infections are often caused by antibiotic resistant bacteria. In some of these cases, prediction of the most effective antibiotic (s) may urgently be needed. This can not be achieved unless the nature and patterns of the resistance to antibiotics among the pathogenic bacteria has been described earlier. The urine samples that reached the laboratory of Shaheed Mustafa K...

Method of Extracting Starch from Bacteria

Development of a simple and sensitive fluorimetric method for isolation of coumaphos-hydrolysing bacteria.

AIMS To develop a simple, rapid and sensitive fluorimetric assay to detect, isolate and characterize a soil bacterium capable of degrading the organophosphorus pesticide, coumaphos. METHODS AND RESULTS A high throughput microtitre plate-based method was used to quantify coumaphos hydrolysis by the bacterium. The fluorescent hydrolysis product of coumaphos, chlorferon, was detected at levels a...

Screening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production

Background: Alginate is a linear polysaccharide consisting of guluronate (polyG) and mannuronate (polyM) subunits. Methods: In the initial screening of alginate-degrading bacteria from soil, 10 isolates were able to grow on minimal medium containing alginate. The optimization of cell growth and alginate lyase (algL) production was carried out by the addition of 0.8% alginate and 0.2-0.3 M NaCl ...