sequence analysis of lysozyme c from the scorpion mesobuthus eupeus venom glands using semi- nested rt-pcr
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Sequence Analysis of Lysozyme C from the Scorpion Mesobuthus Eupeus Venom Glands Using Semi-Nested RT-PCR
BACKGROUND Lysozyme is an antimicrobial protein widely distributed among eukaryotes and prokaryotes and take part in protecting microbial infection. Here, we amplified cDNA of MesoLys-C, a c-type lysozyme from the most common scorpion in Khuzestan Province, Southern Iran. METHODS Scorpions of Mesobuthus eupeus were collected from the Khuzestan Province. Using RNXTM solution, the total RNA was...
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This work aimed to study the characterization and isolation of a alpha neurotoxinBmK, from the venom glands of Scorpion Buthida Mesobuthus eupeus Kuzestan (Iran). Scorpions Mesobuthus eupeus were collected from the Khuzestan province. Using RNXTM solution, the total RNA was extracted from the twenty separated venom glands. cDNA was synthesized with extracted total RNA as template and modified o...
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The venoms of Buthidae scorpions are known to contain basic, single-chain protein -toxins consisting of 60-70 amino acid residues that are tightly cross-linked by four disulfide bridges. Total RNA was extracted from the venom glands of scorpion Mesobuthus eupeus collected from the Khuzestan province of Iran and then cDNA was synthesized with the modified oligo (dT) primer and extracted total R...
متن کاملFirst transcriptome analysis of Iranian scorpion, Mesobuthus eupeus venom gland
Scorpions are generally an important source of bioactive components, including toxins and other small peptides as attractive molecules for new drug development. Mesobuthus eupeus, from medically important and widely distributed Buthidae family, is the most abundant species in Iran. Researchers are interesting on the gland of this scorpion due to the complexity of its venom. Here, we have analyz...
متن کاملFirst transcriptome analysis of Iranian scorpion, Mesobuthus eupeus venom gland
Scorpions are generally an important source of bioactive components, including toxins and other small peptides as attractive molecules for new drug development. Mesobuthus eupeus, from medically important and widely distributed Buthidae family, is the most abundant species in Iran. Researchers are interesting on the gland of this scorpion due to the complexity of its venom. Here, we have analyz...
متن کاملSequence Characterization of cDNA Sequence of Encoding of an Antimicrobial Peptide With No Disulfide Bridge from the Iranian Mesobuthus Eupeus Venomous Glands
BACKGROUND Scorpion venom glands produce some antimicrobial peptides (AMP) that can rapidly kill a broad range of microbes and have additional activities that impact on the quality and effectiveness of innate responses and inflammation. OBJECTIVES In this study, we reported the identification of a cDNA sequence encoding cysteine-free antimicrobial peptides isolated from venomous glands of thi...
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عنوان ژورنال:
iranian red crescent medical journalجلد ۱۳، شماره ۱۰، صفحات ۷۱۹-۷۲۵
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