purification and characterization of the cloned human gm-csf gene expressed in escherichia coli

the human granulocyte-macrophage colony stimulation factor (hgm-csf) gene was cloned in the pet 23a( +) expression vector under the control of strong bacteriophage t7 transcription and translation signals. the hgm-csf gene was transferred into e. coli strainbl21 (de3)plyss andiptg was used for induction of gm-csf gene. production of the target protein was obtained as revealed by elisa and western blot analysis. the produced 'h:gm-csf was purified by immunoaffinity chromatography. the dot blot positive fractions were assayed for biological activity and it was shown that the expressed gm-csf is active.