should we perform semen analysis, dna fragmentation, and hypo-osmotic swelling tests together?
نویسندگان
چکیده
semen analysis, sperm dna fragmentation (sdf) and hypo-osmotic swelling test (host) are usually performed for the evaluation of sperm fertilizing ability. there are some debates over the necessity of sdf and host incorporation in male infertility work-up.semen of 77 men was evaluated by sdf and host through three semen analyses. sperm parameters were arranged into different categories: < 5%, 5-15%, >15% for normal morphology; < 50%, 50-70%, >70 % for motility; and < 10, 10-20, 21-34, 35-50, >50 million/ml for concentration. sdf analysis was performed and values under 30% were assumed to be normal. normal range of host was considered to be >60%.only normal sperm morphology had significant relationship with df rate (p< 0.001). normal morphology, motility, and concentration of sperms had significant relationship with host (p< 0.001, 0.05, and < 0.003,respectively). there was a significant negative correlation between sperm morphology and df rate. the correlations between sperm parameters and percentage of host were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). receiver operating characteristic curve (roc) showed that sperm morphology is a strong predictor of the rate of df and host (accuracy: 0.74‚ and 0.81, respectively). the best sperm morphology cut off point for df and host rate prediction was 4.5% and 5.5%, respectively.sperm morphology had significant correlation with df rate and host and is supposed to be a predictor for these tests. performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases.
منابع مشابه
Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-1...
متن کاملShould We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-1...
متن کاملCorrelation between Different Patterns of Hypo-Osmotic Swelling and Sperm Functional Tests
BACKGROUND Sperm membrane integrity is not only important as a barrier between intra- and extra-cellular spaces, but also it can be considered as a sign of DNA integrity. Hypoosmotic swelling test reflects membrane integrity and has been used to evaluate sperm quality. Intracytoplasmic sperm injection (ICSI) in adjunct with hypo-osmotic swelling test (HOST) has been used for treatment of males ...
متن کاملcorrelation between different patterns of hypo-osmotic swelling and sperm functional tests
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متن کاملThe role of hypo-osmotic swelling test in recurrent abortions.
The role of male factors in abortions has not been studied extensively. We undertook this study to determine if there was any relationship between hypo-osmotic swelling test score and recurrent abortions. This was a cross-sectional case control study conducted at the semen analysis laboratory at a tertiary level referral centre. The male partners of 25 patients who had three or more first trime...
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عنوان ژورنال:
international journal of molecular and cellular medicineجلد ۵، شماره ۴، صفحات ۰-۰
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