Zymophore identification enables the discovery of novel phenylalanine ammonia lyase enzymes
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چکیده
منابع مشابه
Thermal bifunctionality of bacterial phenylalanine aminomutase and ammonia lyase enzymes.
Phenylalanine aminomutases (PAMs) are 4-methylideneimidazol-5-one (MIO)-dependent enzymes that catalyze the isomerization of (S)-a-phenylalanine to give (S)or (R)-bphenylalanine, which are precursors in the biosynthesis of various natural products. Several related tyrosine aminomutases (TAMs) have also been characterized. Furthermore, the mechanistically related MIO-dependent phenylalanine, tyr...
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I?henyialanine ammonia-lyase from the yeast Rhodotorula gluiinis was purified by salt fractionations and Sephadex chromatography. Density gradient centrifugation and Sephadex chromatography indicated its molecular weight to be about 275,000. Enzymatic deamination of several ring-substituted phenylalanine analogues and n-phenylalanine was studied. While cinnamic acid, a product of deamination, a...
متن کاملFungal and Plant Phenylalanine Ammonia-lyase
L-Phenylalanine is one of the essential amino acids that cannot be synthesized in mammals in adequate amounts to meet the requirements for protein synthesis. Fungi and plants are able to synthesize phenylalanine via the shikimic acid pathway. L-Phenylalanine, derived from the shikimic acid pathway, is used directly for protein synthesis in plants or metabolized through the phenylpropanoid pathw...
متن کاملEnzymes in Metabolism of Amino Acids. VII.* Phenylalanine Ammonia-Lyase in Maize {Zea mays L.)
We have isolated an enzyme, phenylalanine ammonia-lyase (EC 4.3.1.5.), from the rootlets of Zea mays var. СЕ-IV. After partial purification by fractional salting out with ammonium sulfate and gel chromatography on Sephadex G-100, we obtained a preparation of a 40-fold greater specific activity than that of the crude homogenate. We hbve determined the ap parent Michaelis constant and the activa...
متن کاملl-Phenylalanine Ammonia-Lyase Activity During Germination of Phaseolus vulgaris.
l-Phenylalanine ammonia-lyase (PAL) activity develops in excised bean axes after approximately 5 hours of incubation and reaches a maximum level after 14 hours of incubation. Light does not affect the development of activity, but puromycin, cycloheximide, actinomycin D, and 5-fluorouracil inhibit.During this period of incubation both d- and l-p-fluorophenylalanine stimulate fresh weight increas...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-13990-0