Zinc binding reverses the calcium-induced arachidonic acid-binding capacity of the S100A8/A9 protein complex

THE CALCIUM BINDING SITES OF THE BAKERS' YEAST TRANSKETOLASE

The calcium binding sites of Bakers' Yeast Transketolase (TK) was elucidated by estimating the pKa values of the functional groups that bind to calcium. These pKa's were found to be 6.25 and 7.2 relating to the pKa's of the two immidazol moieties of histidine residues on the enzyme. The rate of the binding of calcium to the enzyme was obtained separately as a function of pH. Maximum values ...

Competition of zinc, cadmium and calcium for binding sites in sperm of trout and carp

Heavy metal pollutions of aquatic ecosystems have already raised many concerns on aquaticorganisms’health and survival especially on their sperm. The effects of a single metal on sperm may be totallydifferent from cocktail of several metals because of their interactions, so the effects of zinc, cadmium andcalcium on the trout and carp sperms and their competition have been examined by incubatio...

Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22.

By yeast two-hybrid screening using the calcium-binding protein ALG-2 as bait a new target of ALG-2 was identified, the RNA-binding protein RBM22. In order to confirm these interactions in vivo we prepared fluorescent constructs by using the monomeric red fluorescent protein to label ALG-2 and the enhanced green fluorescent protein to label RBM22. Confocal microscopy of NIH 3T3 cells transfecte...

Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy

molecular aspect on the interaction of zinc-ofloxacin complex with deoxyribonucleic acid, proposed model for binding and cytotoxicity evaluation

recently, several studies have shown that the metal-fluoroquinolone complexes have more antibacterial and cytotoxic effects in comparison with free fluoroquinolones. these results may introduce new drugs for chemotherapy with fewer side effects. in this work a bidentated zinc (ii) complex with ofloxacin (ozc) was synthesized and cytotoxicity activities and dna binding of the resulted complex wa...