Zebrafish miR-462-731 regulates hematopoietic specification and pu.1-dependent primitive myelopoiesis

Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis.

In vertebrates, myeloid cells comprise polymorphonuclear and mononuclear lineages that arise from 2 successive waves of development: a transitory primitive wave giving rise to limited myeloid cells during embryonic stage and a definitive wave capable of producing myeloid cells throughout the fetal and adult life. One key unresolved question is what factors dictate polymorphonuclear versus monon...

Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis Running Head: irf8 in primitive myeloid cell development Key Words: Zebrafish, Primitive Myelopoiesis, Macrophages, Neutrophils, Irf8

Maternal pak4 expression is required for primitive myelopoiesis in zebrafish

Transcripts of pak4, the zebrafish ortholog of p21-activated kinase 4 (PAK4), are most abundant in the egg and fall to low levels by the end of gastrulation, after which expression is essentially ubiquitous. Translation of maternal mRNA into pak4 protein is first detectable at high stage (3.3hpf). Splice-blocking morpholino oligonucleotides (MOs) were used to prevent zygotic pak4 expression. Th...

MiR-145 regulates hematopoiesis during zebrafish development

MicroRNA-145 (miR-145) is considered to play key role in many cellular processes, such as proliferation, differentiation and apoptosis by inhibiting target gene expression. The transcriptional regulation of cardiovascular development requires precise spatiotemporal control of gene expression. We are beginning to understand the functions of miRNA-145 played during essential biological processes ...

miR-34a Regulates Sperm Motility in Zebrafish

Increasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knoc...