Yunus Emre Bibliyografyas? (1991-2021)

Asymmetric protonation of EmrE.

The small multidrug resistance transporter EmrE is a homodimer that uses energy provided by the proton motive force to drive the efflux of drug substrates. The pKa values of its "active-site" residues--glutamate 14 (Glu14) from each subunit--must be poised around physiological pH values to efficiently couple proton import to drug export in vivo. To assess the protonation of EmrE, pH titrations ...

Radiologic Imaging in Chronic Sinusitis

Sinusitis is one of the most common diseases which primary care physicians encounter in their daily practice [1]. Nowadays, it’s widely approved that radiologic imaging is not indicated to evaluate uncomplicated sinusitis cases. Further diagnostic evaluation and radiologic imaging should be reserved for sinusitis cases whose symptoms persist or recurr despite appropriate treatment. Sinusitis ca...

EMBL Programme 2017-2021: Digital Biology

Parallel topology of genetically fused EmrE homodimers.

EmrE is a small H+-coupled multidrug transporter in Escherichia coli. Claims have been made for an antiparallel topology of this homodimeric protein. However, our own biochemical studies performed with detergent-solubilized purified protein support a parallel topology of the protomers. We developed an alternative approach to constrain the relative topology of the protomers within the dimer so t...

New free-exchange model of EmrE transport.

EmrE is a small multidrug resistance transporter found in Escherichia coli that confers resistance to toxic polyaromatic cations due to its proton-coupled antiport of these substrates. Here we show that EmrE breaks the rules generally deemed essential for coupled antiport. NMR spectra reveal that EmrE can simultaneously bind and cotransport proton and drug. The functional consequence of this fi...