Yüksek infektif titre ve 146S FMD virus partikülü eldesi için uygun BHK-21 hücre kültürü hatlarının saptanması

Foot and mouth disease (FMD) is one the most contagious diseases in cloven-hoofed animals has a great potential for causing severe economic loss susceptible animals. In fight against FMD disease, inactive vaccines are widely used. This study was carried out detection of suitable BHK-21 cell lines order to get virus suspensions containing excess amounts 146S serotypes O, A Asia-1, vaccine production. For this purpose, addition An30 culture currently used production, An73 were passaged 20 times. every 5 passages, infective titers particle quantities Asia-1 measured. Additionally, karyotype analysis performed on both cells at 20th passage levels tests revealed that there no significant change morphological features chromosomal structures cells.
 It determined titer very high line than they available line. The increases statistically (p