YTHDC2 inhibits rat bone mesenchymal stem cells osteogenic differentiation by accelerating RUNX2 mRNA degradation via m6A methylation

As the most abundant internal mRNA modification, N6-methyladenosine (m6A) RNA methylation has been found to influence many biological events including bone mesenchymal stem cells (BMSCs) osteogenic differentiation. YTH binding protein C2 （YTHDC2） is an m6A reading with ability mediate decay of combined methylated mRNA, however its role in BMSCs differentiation remains unknown. In this study, we first increase RUNX family transcription factor 2 （RUNX2） expression and a decrease YTHDC2 during process Furthermore, transfected interference fragment, resulting increased content RUNX2 inside BMSCs. Finally, through Immunoprecipitation experiments, confirmed that can bind accelerate decomposition. Moreover, immunofluorescence staining also showed negative correlation between RUNX2. conclusion, differentiation, decreased expression, higher level (mRNA protein) cells, indicating as promising molecular target for regulation