منابع مشابه
Mechanisms of YidC-mediated insertion and assembly of multimeric membrane protein complexes.
The YidC protein fulfills a dual and essential role in the assembly of inner membrane proteins in Escherichia coli. Besides interacting with transmembrane segments of newly synthesized membrane proteins that insert into the membrane via the SecYEG complex, YidC also functions as an independent membrane protein insertase and assists in membrane protein folding. Here, we discuss the mechanisms of...
متن کاملYidC-mediated membrane insertion of assembly mutants of subunit c of the F1F0 ATPase.
YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F(1)F(0) ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c un...
متن کاملSec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC.
Recent studies identified YidC as a novel membrane factor that may play a key role in membrane insertion of inner membrane proteins (IMPs), both in conjunction with the Sec-translocase and as a separate entity. Here, we show that the type II IMP FtsQ requires both the translocase and, to a lesser extent, YidC in vivo. Using photo-crosslinking we demonstrate that the transmembrane (TM) domain of...
متن کاملSubunit a of cytochrome o oxidase requires both YidC and SecYEG for membrane insertion.
The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that facilitate the insertion of membrane proteins. Depletion of YidC in E. coli leads to a specific defect in the functional assembly of major energy transducing complexes such as the F1F0 ATPase and cytochrome bo3 oxidase. Here we report on the in vitro reconstitution of the membrane insertion of the CyoA subuni...
متن کاملReal Time Observation of Single Membrane Protein Insertion Events by the Escherichia coli Insertase YidC
Membrane protein translocation and insertion is a central issue in biology. Here we focus on a minimal system, the membrane insertase YidC of Escherichia coli that inserts small proteins into the cytoplasmic membrane. In a reconstituted system individual insertion processes were followed by single-pair fluorescence resonance energy transfer (FRET), with a pair of fluorophores on YidC and the su...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2018
ISSN: 1574-6968
DOI: 10.1093/femsle/fny106