Yarrowia lipolytica lipase production enhanced by increased air pressure

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Yarrowia lipolytica lipase production enhanced by increased air pressure.

AIMS To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultures at atmospheric pressure. No inhibition of cellular growth was observed by...

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The production of Yarrowia lipolytica lipase powder by improved spray-drying method

Lipase is used in the production of foods, flavor enhancers, detergents, cosmetics and pharmaceuticals. A common impediment to the production of commercial enzymes is their low-stability aqueous solutions. In this study, the downstream process was investigated to obtain a stable spray-dried lipase powder of Yarrowia lipolytica. The enzyme solution samples were supplemented with different concen...

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Impact of Lipase-Mediated Hydrolysis of Castor Oil on c-Decalactone Production by Yarrowia lipolytica

c-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the c-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L was found to ...

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Improving the thermostability of lipase Lip2 from Yarrowia lipolytica.

Yarrowia lipolytica lipase Lip2 (YlLip2) is a highly versatile biocatalyst. However, its practical use is often hampered by its low stability. Here three complementary protein engineering strategies were used to improve the thermostability of this enzyme. The first strategy was error-prone PCR based directed evolution, which resulted in a YlLip2 variant with a 2.5-fold longer half-life of therm...

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Characterization of an Extracellular lipase from Yarrowia lipolytica

Lipases from different sources have distinct properties, such as specificity, stability and optimal operational conditions. Moreover, different conditions of lipase production may alter these enzyme properties. Therefore, the aims of this study were related to the evaluation of lipase activity of Yarrowia lipolytica IMUFRJ 50682 (isolated from Baía de Guanabara, Brazil) produced under submerged...

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ژورنال

عنوان ژورنال: Letters in Applied Microbiology

سال: 2007

ISSN: 0266-8254,1472-765X

DOI: 10.1111/j.1472-765x.2007.02299.x