Xylulose and glucose fermentation by Saccharomyces cerevisiae in chemostat culture

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Xylulose and glucose fermentation by Saccharomyces cerevisiae in chemostat culture.

Saccharomyces cerevisiae ATCC 24860 was cultivated in chemostat culture under anoxic conditions with 111.1 mmol of glucose liter-1 alone or with a mixture of 66.7 mmol of xylulose liter-1 and 111.1 mmol of glucose liter-1. The substrate consumption rate was 5.4 mmol g of cells-1 h-1 for glucose, whereas for xylulose it was 1.0 mmol g of cells-1 h-1. The ethanol yield decreased from 0.52 carbon ...

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A dynamic flux balance model and bottleneck identification of glucose, xylose, xylulose co-fermentation in Saccharomyces cerevisiae.

A combination of batch fermentations and genome scale flux balance analysis were used to identify and quantify the rate limiting reactions in the xylulose transport and utilization pathway. Xylulose phosphorylation by xylulokinase was identified as limiting in wild type Saccharomyces cerevisiae, but transport became limiting when xylulokinase was upregulated. Further experiments showed xylulose...

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Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures.

For ethanol production from lignocellulose, the fermentation of xylose is an economic necessity. Saccharomyces cerevisiae has been metabolically engineered with a xylose-utilizing pathway. However, the high ethanol yield and productivity seen with glucose have not yet been achieved. To quantitatively analyze metabolic fluxes in recombinant S. cerevisiae during metabolism of xylose-glucose mixtu...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1996

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.62.5.1705-1709.1996