Xylella fastidiosagenotype differentiation by SYBR®Green-based QRT-PCR

DEVELOPMENT OF QRT-PCR PROTOCOLS FOR RAPID XYLELLA FASTIDIOSA SUBSPECIES DIAGNOSTICS Principal Investigator:

Xylella fastidiosa (Xf) is a plant pathogenic bacterium that is transmitted between hosts by the glassy-winged sharpshooter (GWSS; Homalodisca vitripennis). Multiple subspecies of Xf occur and are host specific. Xf subsp. fastidiosa is the causal agent of Pierce’s disease (PD). Xf subsp. multiplex and Xf subsp. sandyi are commonly found in North America but do not cause PD. Rapid diagnostics to...

Evaluation of bacterial internalization using qRT-PCR.

1Bacterial resistance to antibiotics currently represents a topic of high interest. Unfortunately, studies addressing their adaptation and multidrug tolerance latency are not sufficient for a comprehensive description of the pathogen-host molecular interaction mechanisms (interactome) and the bacterial internalization into human cells, which leads to modified human genes. Bacterial persisters a...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Evaluating L1CAM expression in human endometrial cancer using qRT-PCR

BACKGROUND Management of endometrial carcinoma (EC) still needs improvement of risk assessment. Recently, L1CAM immunohistochemical (IHC) evaluation showed a unique value to predict the outcome of early EC. However IHC results are often conflicting for lack of inter-laboratory standardisation. METHODS Here, as a proof of concept and to increase reproducibility we assayed eighty-two EC and 26 ...

No Control Genes Required: Bayesian Analysis of qRT-PCR Data

BACKGROUND Model-based analysis of data from quantitative reverse-transcription PCR (qRT-PCR) is potentially more powerful and versatile than traditional methods. Yet existing model-based approaches cannot properly deal with the higher sampling variances associated with low-abundant targets, nor do they provide a natural way to incorporate assumptions about the stability of control genes direct...