Xylanase production by Trichoderma virens MLT2J2 under solid-state fermentation using corn cob as a substrate

Abstract. Istiqomah L, Cahyanto MN, Zuprizal. 2022. Xylanase production by Trichoderma virens MLT2J2 under solid-state fermentation using corn cob as a substrate. Biodiversitas 23: 6530-6538. is one of non-starch polysaccharide (NSP) degrading enzyme encompass in industrial application like animal feed, food, biofuel, and textile. The objective this study was to produce characterize the crude xylanase from isolate that isolated coconut husk. Molecular identification revealed identified virens. produced (108 spores/g corncob) substrate with 80% initial moisture content (SSF) at 30°C for 7 d incubation. Changes surface morphology structure fermented were monitored Scanning Electron Microscope (SEM). Statistical analysis performed One-way variance (ANOVA) followed Tukey Kramer post hoc test compare treatment means. Under parameters maximum activity 181.22 U/g-IDW, loss dry matter 11.43%, pH decreased (4.11) 5 incubation compared (5.47). between broad range 3-8 showed acidophilic mesophilic characteristics (optimum 5,0 40°C), also conserved more than 50% before 4 h after 40°C. micrographs SSF appeared intact smoother, while turned coarser SSF, suggestive disrupted porous surface. These findings reveled T. could extracellular potential disrupt cell wall cheap thereby increased reactive area enzymatic situ hydrolysis.