منابع مشابه
Iodination of nucleic acids in organic solvents with iodine monochloride.
Iodine monochloride, labeled with 1z51, iodinated ribonucleic acid and deoxyribonucleic acid dissolved as hexadecyltrimethylammonium salts in anhydrous dimethylformamide or N-ethyl acetamide. In the case of RNA (polyuridylate, polycytidylate, and soluble RNA) 5-iodouridine S/-phosphate, 8-iodoguanosine 5’-phosphate, and 5-iodocytidine 5’-phosphate are found. After 24 hours of reaction, 32% of U...
متن کاملHigh Specific Activity Iodination of v-Globulin with Iodine-131 Monochloride*
A technic, efficient in use ~ of I TM, has been developed for introducing I TM into 4-mg. amounts of antibody or 3,-globulin protein at a level of 10 me. I ~al per mg. protein with a resulting iodine content of ~-S atoms per protein molecule of 160,000 molecular weight. Oak Ridge I TM was adjusted to pH 8 with borate buffer and the requisite amount of hydrochloric acid. To this solution was add...
متن کاملSome quantitative aspects of the labelling of proteins with 125 I by the iodine monochloride method.
The labelling of proteins by the iodine monochloride method was studied by using a mathematical model. The equations used were primarily derived from the mass law equation of the isotopic exchange reaction between [(125)I]iodide and iodine monochloride. For convenient application, all equations were programmed into a computing desk-top calculator. To support the validity of the theoretical mode...
متن کاملRadioiodinated fatty acids for heart imaging: iodine monochloride addition compared with iodide replacement labeling.
Radioiodinated fatty acids have been proposed as agents for use in heart imaging. Previous studies in experimental animals and humans using 131I-oleic acid of low specific activity were marginally successful. Higher specific activity compounds offer potential improvement for use as imaging agents for normal myocardium. Methods for preparation of high specific activity, radioiodinated fatty acid...
متن کاملThe mechanism of inactivation of glyceraldehyde 3-phosphate dehydrogenase by tetrathionate, o-iodosobenzoate, and iodine monochloride.
The reversible and irreversible inactivation of pig muscle glyceraldehyde j-phosphate dehydrogenase (n-glyceraldehyde-d-phosphate:nicotinamide adenine dinucleotide oxidoreductase (phosphorylating), EC 1.2.1.12) with tetrathionate, o-iodosobenzoate, and iodine monochloride has been studied. This investigation has revealed that tetrathionate reacts stoichiometrically with the catalytically active...
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ژورنال
عنوان ژورنال: J. Chem. Soc.
سال: 1873
ISSN: 0368-1769
DOI: 10.1039/js8732600815