Xenopus laevisCu,Zn superoxide dismutase B cDNA sequence

Superoxide dismutase Superoxide dismutase

cDNA and deduced amino acid sequence of murine Cu-Zn superoxide dismutase.

Cu-Zn superoxide dismutase (EC 1.15.1.1) is a eukaryotic cytoplasmic enzyme involved in the antioxidant defense against the toxic effects of superoxide anion-induced cellular damage (1). We have isolated cDNA clones from a murine SWR/J liver cDNA library (2) using the human cDNA clone pS61-10 as a heterologous probe (3). The nucleotide and deduced amino acid sequence of one of these clones cont...

Xenopus laevis ribosomal protein S1a cDNA sequence.

In Xenopus laevis the coordinated synthesis of the over 80 ribosomal proteins (r-proteins) is attained by both post-transcriptional and translational level regulation (1, 2). Also several r-protein genes analyzed present structural similarities probably involved in the common regulatory mechanisms. Our understanding, however, of the structure and expression of these genes is based on the analys...

The amino acid sequence of mangano superoxide dismutase from Escherichia coli B.

The complete amino acid sequence of the mangano superoxide dismutase from Escherichia coli B has been deduced through characterization of peptides from cyanogen bromide, bromonitrophenylsulfenyl skatole, citraconylated tryptic, and succinylated tryptic digests of the intact polypeptide chain and through subfragmentation of selected peptides with chymotrypsin, thermolysin, trypsin, and Staphyloc...

Sequence and structural determinants of Cu, Zn superoxide dismutase aggregation.

Diverse point mutations in the enzyme Cu, Zn superoxide dismutase (SOD1) are linked to its aggregation in the familial form of the disease amyotrophic lateral sclerosis. The disease-associated mutations are known to destabilize the protein, but the structural basis of the aggregation of the destabilized protein and the structure of aggregates are not well understood. Here, we investigate in sil...