X-Ray Structure Analysis of [NiFe] Hydrogenase.
نویسندگان
چکیده
منابع مشابه
Crystal Structure Analysis of [NiFe] Hydrogenase Maturation Proteins
Introduction [NiFe]-hydrogenases harbor a complex metal cofactor, NiFe(CN)2CO, in their active sites. Its biosynthesis requires specific maturation machinery, in which six Hyp proteins (HypABCDEF) play key roles. Four Hyp proteins (HypCDEF) are involved in the biosynthesis and incorporation of the Fe(CN)2CO group. After Fe insertion, HypA and HypB insert the Ni ion into the hydrogenase large su...
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Hydrogenase maturation endopeptidases catalyse the terminal step in the maturation of the large subunit of [NiFe]-hydrogenases. They remove a C-terminal extension from the precursor of the subunit, triggering a conformational switch that results in the bridging of the Fe and Ni atoms of the metal centre via the thiolate of a cysteine residue and in closure of the centre. This review summarizes ...
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In this work, structural and magnetic properties of LaMnO3+δ compound prepared by citrate precursor method and annealed in presence of oxygen are investigated. The structural characterization of LaMnO3+δ by X-ray powder diffraction and using X’pert package and Fullprof program is evidence for a rhombohedral structure (R-3c space group) confirmed by FTIR measurement. The magnetic measurements sh...
متن کاملHow the structure of the large subunit controls function in an oxygen-tolerant [NiFe]-hydrogenase
Salmonella enterica is an opportunistic pathogen that produces a [NiFe]-hydrogenase under aerobic conditions. In the present study, genetic engineering approaches were used to facilitate isolation of this enzyme, termed Hyd-5. The crystal structure was determined to a resolution of 3.2 Å and the hydro-genase was observed to comprise associated large and small subunits. The structure indicated t...
متن کاملStructure of [NiFe] hydrogenase maturation protein HypE from Escherichia coli and its interaction with HypF.
Hydrogenases are enzymes involved in hydrogen metabolism, utilizing H2 as an electron source. [NiFe] hydrogenases are heterodimeric Fe-S proteins, with a large subunit containing the reaction center involving Fe and Ni metal ions and a small subunit containing one or more Fe-S clusters. Maturation of the [NiFe] hydrogenase involves assembly of nonproteinaceous ligands on the large subunit by ac...
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ژورنال
عنوان ژورنال: Nihon Kessho Gakkaishi
سال: 2001
ISSN: 0369-4585,1884-5576
DOI: 10.5940/jcrsj.43.227