X-ray scattering from unilamellar lipid vesicles
نویسندگان
چکیده
منابع مشابه
Structure of fully hydrated fluid phase DMPC and DLPC lipid bilayers using X-ray scattering from oriented multilamellar arrays and from unilamellar vesicles.
Quantitative structures of the fully hydrated fluid phases of dimyristoylphosphatidylcholine (DMPC) and dilauroylphosphatidylcholine (DLPC) were obtained at 30 degrees C. Data for the relative form factors F(q(z)) for DMPC were obtained using a combination of four methods. 1), Volumetric data provided F(0). 2), Diffuse x-ray scattering from oriented stacks of bilayers provided relative form fac...
متن کاملModels to analyze small-angle neutron scattering from unilamellar lipid vesicles.
Small-angle scattering has been employed to study the structure of lipid bilayers in unilamellar vesicles. This paper evaluates the use of a model approach for the analysis of such data. A long molecular dynamics simulation of a dipalmitoylphosphatidylcholine bilayer in the L(alpha) phase provides detailed structural information from which scattering length density profiles and scattering inten...
متن کاملJoint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles
Low- and high-resolution models describing the internal transbilayer structure of asymmetric lipid vesicles have been developed. These models can be used for the joint analysis of small-angle neutron and X-ray scattering data. The models describe the underlying scattering length density/electron density profiles either in terms of slabs or through the so-called scattering density profile, previ...
متن کاملMaking giant unilamellar vesicles via hydration of a lipid film.
This unit describes protocols for making giant unilamellar vesicles (GUVs) based on rehydration of dried lipid films. These model membranes are useful for determining the impact of membrane and membrane-binding components on lipid bilayer stiffness and phase behavior. Due to their large size, they are especially amenable to studies using fluorescence and light microscopy, and may also be manipu...
متن کاملA Stratified Flow-driven Route to Monodisperse Unilamellar Lipid Vesicles
Giant unilamellar vesicles (GUVs) are good models of living cells, owing to their size and lamellarity. Compartmentalization within lipid vesicles has been exploited for the study of membrane behavior, lipid mechanics and a variety of biological processes, though their synthesis is not straightforward. We describe the development of a stratified flow driven microfluidic approach to GUV assembly...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Applied Crystallography
سال: 2005
ISSN: 0021-8898
DOI: 10.1107/s0021889804029206