Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.)

This study investigated the antioxidant enzyme activities in skin mucus of gilthead seabream (Sparus aurata L.) at 3 and 7 days post-wounding (dpw). The expression levels genes that encode stress proteins (grp170, grp94, grp75, sod hsp70) regeneration-related (tf, igf1, tgfb1, der1, apo1 erdj3) also were determined. Mucus samples obtained from left right flanks non-wounded wounded fish. In both fish, catalase glutathione reductase increased (p < 0.05) dpw (100 ± 31% 111 25%, respectively), whereas superoxide dismutase activity only (135 15%). flank fish mainly (grp170 to 288 85%, grp94 502 143%, grp75 274 69%, 569 99%, hsp70 537 14%) 0.05). However, tissue varied depending on investigated, experimental time, gene studied. To best our knowledge, this is first work determine effect a wound different parts same