Wound Healing: Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown) (Adv. Biology 5/2021)
نویسندگان
چکیده
In article number 2000307, Tim Blomeier and co-workers describe the Blue Light-Operated CRISPR/Cas13b-mediated mRNA Knockdown. “Lockdown” introduces optical control of RNA levels utilizing a blue light-dependent switch to induce expression CRISPR/Cas13b, which mediates sequence-specific knockdown exo- endogenous mRNA. Further, they combined tool with other optogenetic switches for efficient downregulation target proteins in mammalian cells.
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ژورنال
عنوان ژورنال: Advanced biology
سال: 2021
ISSN: ['2701-0198']
DOI: https://doi.org/10.1002/adbi.202170051