Wogonin Stimulation of Cell Death and Reducing Survivin in MDM-MB231 Breast Tumors

Background: Wogonin as a flavonoid compound is known for its anticancer effect through growth control, differentiation, and apoptosis. This study investigates the possible activity of wogonin in inducing cell death breast tumors MDA-MB231. Materials Methods: The level proliferation was evaluated by MTT assay. Cycle analysis apoptotic rate were assessed flow cytometry. mRNA Bax, Bcl-2, p53, survivin real-time PCR. Western blot utilized to assert relative protein expression. Results: inhibits MDA-MB231 over time, particular, can induce arrest phase G1 cells. apoptosis related remarkable decrease Bcl-2 an increment p53 Bax. also significantly elevated active forms caspase-3, -8, -9. Z-DEVD-FMK, specific inhibitor remarkably cellular caused wogonin. suppresses PI3K/Akt phosphorylation ERK-derived phosphorylation. PD98059, ERK inhibitor, blocked Also, LY294002, suppressor PI3K, elevates Various studies demonstrated that LY294002 not only modulates expression gene alone but enhances suppression with Conclusion: role observed triggering caspases associated suppressive pathways PI3K/Akt/survivin signal