Widefield Multiphoton Imaging at Depth with Temporal Focusing

Abstract Optical imaging has the potential to reveal high-resolution information with minimal photodamage. The recent renaissance of super-resolution, widefield, ultrafast, and computational methods broadened its horizons even further. However, a remaining grand challenge is at depth over widefield high spatiotemporal resolution. This achievement would enable observation fast collective biological processes, particularly those underpinning neuroscience developmental biology. Multiphoton depth, combining temporal focusing single-pixel detection, an emerging avenue address this challenge. novel physics driving approach offer great for future advances. chapter articulates theories detection details specific TempoRAl Focusing microscopy single-pIXel (TRAFIX), particular focus on current practical implementation prospects.