Whey and post-frying oil as substrates in the process of microbial lipids obtaining: a value-added product with nutritional benefits

Abstract Yarrowia lipolytica has found many biotechnological applications. The species a number of regulatory mechanisms to maintain cellular homeostasis, enabling biomass growth in complex media. aim this study was evaluate the use Y. yeast as platform for simultaneous management several industrial by-products and production microbial lipids with application potential chemical food industries. Batch cultures KKP 379 strain were conducted media post-frying rapeseed oil (PFO) by-product curd cheese production—acid whey. To nutraceutical, quantitative qualitative analyses sterols carried out along an assessment mineral composition. It indicated that composition content varied depending on phase cell batch culture. During culture medium 20% (v/v) whey 50 g/L PFO, lipid reached 39% (w/w). highest amount per dry (7.38 mg/g) (21.08 recorded after 38 h dominant ergosterol 12.10 mg/g (57%). In addition, carbon nitrogen sources affected selected elements biomass, indicating substrate modification can be tool manipulating cells. results showed selection waste substrates is important factor regulation accumulation efficiency, well certain sterols.