منابع مشابه
What is broad-range 16S rDNA PCR?
Patel A, et al. Arch Dis Child Educ Pract Ed 2017;0:1–4. doi:10.1136/archdischild-2016-312049 INTRODUCTION PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s. Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nuclei...
متن کاملDevelopment of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service.
The aim of the present study was to develop a broad-range PCR based on bacterial 16S rDNA for use in the routine diagnostic clinical microbiology service. The optimization and validation of the assay for use on clinical specimens from normally sterile sites is described, and preliminary results are reported on the use of the assay in the clinical diagnosis of bacterial infection in 382 paediatr...
متن کاملEmpyema: the use of broad range 16S rDNA PCR for pathogen detection.
BACKGROUND An increase in the incidence of thoracic empyema in children has been reported. The causative pathogen is often unknown as pleural fluid is frequently sterile at the time of culture. The role of unusual organisms is unclear. AIMS (1) To compare the detection of organisms in pleural fluid from children with empyema using a molecular technique (16S rDNA polymerase chain reaction (PCR...
متن کاملIdentification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.
DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
متن کاملComparison of broad range 16S rDNA PCR and conventional blood culture for diagnosis of sepsis in the newborn: a case control study
BACKGROUND Early onset bacterial sepsis is a feared complication of the newborn. A large proportion of infants admitted to the Neonatal Intensive Care Unit (NICU) for suspected sepsis receive treatment with potent systemic antibiotics while a diagnostic workup is in progress. The gold standard for detecting bacterial sepsis is blood culture. However, as pathogens in blood cultures are only dete...
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ژورنال
عنوان ژورنال: Archives of disease in childhood - Education & practice edition
سال: 2017
ISSN: 1743-0585,1743-0593
DOI: 10.1136/archdischild-2016-312049