What do we need to obtain high quality circulating tumor DNA (ctDNA) for routine diagnostic test in oncology? – Considerations on pre-analytical aspects by the IFCC workgroup cfDNA

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چکیده

• Preanalytical variables influence the quality and quantity of isolated cfDNA. This paper compares preanalytical aspects published for cfDNA analysis. Blood drawing, collection tube, centrifugation, storage isolation are crucial. The analysis circulating cell free DNA is an important tool tumor resistance, heterogeneity, detection minimal residual disease allograft rejection in kidney or heart transplant patients. proper use this technique important, starts with considering pre-analytic aspects. current addresses some technical considerations to ensure harmonized techniques.

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ژورنال

عنوان ژورنال: Clinica Chimica Acta

سال: 2021

ISSN: ['1873-3492', '0009-8981']

DOI: https://doi.org/10.1016/j.cca.2021.05.033