Western immunoblotting in avian shell gland sample immunoblotting methods
نویسندگان
چکیده
منابع مشابه
Western immunoblotting in avian shell gland sample immunoblotting methods.
Avian shell gland tissue was subjected to Western blot analysis using anti-human estrogen receptor-alpha antibody H222. Initial attempts to obtain consistent, high-quality blots were unsuccessful because, as it turned out, excessive lipid in tissue preparations interfered with protein separation. Incremental additions of acetone eventually proved to be the critical step in solubilizing lipids a...
متن کاملWestern immunoblotting for Bartonella endocarditis.
To differentiate infectious endocarditis (IE) from other Bartonella infections and to identify infecting Bartonella bacteria at the species level on a serological basis, we used Western immunoblotting to test sera from 51 patients with Bartonella IE (of which 27 had previously benefited from species identification by molecular techniques), 11 patients with chronic Bartonella quintana bacteremia...
متن کاملSpecificity of immunoblotting analyses in eosinophilic meningitis.
Angiostrongylus cantonensis and Gnathostoma spinigerum are the two most common causative parasites of eosinophilic meningitis (EOM). Serological tests are helpful tools for confirming the identity of the pathogen. Recent reports determined the specificity of such tests by using normal healthy controls. There have been limited studies done to rule out the cross-reactivity between these two causa...
متن کاملimmunological diagnosis of human hydatid cyst using western immunoblotting technique
background: echinococcosis is a parasitic disease with worldwide distribution which is caused by the tapeworms echinococcus granulosus . diagnosis of the disease relies on imaging techniques, but the techniques are not able to differentiate the cyst from benign or malignant tumors; hence, appropriate serologic methods are required for the differential diagnosis of the infection. materials and m...
متن کاملEnhancement of diaminobenzidine colorimetric signal in immunoblotting.
Immunodetection methods such as immunohistochemistry and immunoblotting (Western blot analysis) are used to specifically and sensitively detect proteins of interest. In both immunohistochemical and immunoblotting techniques, a primary antibody binds to the protein of interest, and through the subsequent detection of this immunoglobulin with a conjugated secondary antibody, the presence of the p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Poultry Science
سال: 2004
ISSN: 0032-5791
DOI: 10.1093/ps/83.2.230