Western immunoblot analysis of protein antigens ofPenicillium marneffei
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چکیده
منابع مشابه
Western immunoblot analysis of the antigens of Haemobartonella felis with sera from experimentally infected cats.
Cats were experimentally infected with a Florida isolate of Haemobartonella felis in order to collect organisms and evaluate the immune response to H. felis. Cryopreserved organisms were thawed and injected intravenously into nonsplenectomized and splenectomized cats. Splenectomized animals were given 10 mg of methylprednisolone per ml at the time of inoculation. Blood films were evaluated dail...
متن کاملWestern blot (immunoblot) analysis of the fimbrial antigens of Bacteroides nodosus.
The roles of the fimbrial subunit and the putative basal protein antigens in the serological classification of Bacteroides nodosus have been examined by Western blot (immunoblot)-antibody binding studies of fimbriae isolated from a wide range of strains representative of different serogroups and serotypes. Fimbrial subunits were recognized by antiserum against the homologous serogroup but not g...
متن کاملImmunoblot detection of antigens in immunoprecipitates.
Co-immunoprecipitation of antigens from cell or tissue extracts is used in a wide variety of experimental systems to corroborate the existence of authentic protein-protein interactions in vivo. Typically, stable protein-protein associations are detected by immunoprecipitation with antibodies specific for one protein, followed by immunoblot analysis with antibodies against potential interacting ...
متن کاملImmunoblot analysis.
Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. It involves the solubilization and electrophoretic separation of proteins, glycoproteins, or lipopolysaccharides by gel electrophoresis, followed by quantitative transfer and irreversible binding to...
متن کاملpotential use of biomphalaria alexandrina snail antigens for serodiagnosis of schistosomiasis mansoni by immunoblot analysis.
background: the aim of this study was to evaluate the possible use of biomphalaria alexandrina snail antigens in diagnosis of schistosomiasis mansoni using enzyme linked immunolectrotransfere blot (eitb). methods: s. mansoni adult worm crude antigens (awa), feet and visceral humps of b. alexandrina and bulinus truncatus were used. hyperimmune mice sera (his) versus each antigen were prepared fo...
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ژورنال
عنوان ژورنال: Medical Mycology
سال: 1997
ISSN: 1369-3786,1460-2709
DOI: 10.1080/02681219780001011