Volume-regulated Cl− current: contributions of distinct Cl− channels and localized Ca2+ signals
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چکیده
منابع مشابه
Activation of Ca2+‐activated Cl− channel ANO1 by localized Ca2+ signals
Ca(2+)-activated chloride channels (CaCCs) regulate numerous physiological processes including epithelial transport, smooth muscle contraction and sensory processing. Anoctamin-1 (ANO1, TMEM16A) is a principal CaCC subunit in many cell types, yet our understanding of the mechanisms of ANO1 activation and regulation are only beginning to emerge. Ca(2+) sensitivity of ANO1 is rather low and at ne...
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Cl channels are membrane proteins that are responsible for the passive flow of Cl into and out of the cell. The Ca-activated Cl channels (CaCCs) are attracting a lot of attention lately. The molecular identity of these channels remained elusive (for a review, see Hartzell et al., 2005) until anoctamin 1 and anoctamin 2 (in mammals ANO1 and ANO2, respectively; in Xenopus TMEM16A and TMEM16B, res...
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Ca(2+)-activated Cl channels (Cl(Ca)Cs) are an important class of anion channels that are opened by increases in cytosolic [Ca(2+)]. Here, we examine the mechanisms of anion permeation through Cl(Ca)Cs from Xenopus oocytes in excised inside-out and outside-out patches. Cl(Ca)Cs exhibited moderate selectivity for Cl over Na: P(Na)/P(Cl) = 0.1. The apparent affinity of Cl(Ca)Cs for Cl was low: K(...
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Chloroquine (CQ), a bitter tasting drug widely used in treatment of malaria, is associated gastrointestinal side effects including nausea or diarrhea. In the present study, we investigated the effect of CQ on electrolyte transport in rat ileum using the Ussing chamber technique. The results showed that CQ evoked an increase in short circuit current (ISC) in rat ileum at lower concentration (≤5×...
متن کاملInfluence of K-Cl cotransporter activity on activation of volume-sensitive Cl- channels in human osteoblasts.
The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl- channels expressed in these cells as described previously. The reversal potential of the induced Cl- current is more negative when act...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Cell Physiology
سال: 2019
ISSN: 0363-6143,1522-1563
DOI: 10.1152/ajpcell.00507.2018