Volume-activated DIDS-sensitive whole-cell chloride currents in trout red blood cells

Volume-activated chloride currents in interstitial cells of Cajal.

Interstitial cells of Cajal (ICC) undergo marked morphological changes on contraction of the musculature, making it essential to understand properties of mechanosensitive ion channels. The whole cell patch-clamp technique was used to identify and to characterize volume-activated Cl- currents in ICC cultured through the explant technique. Hypotonic solutions (approximately 210 mosM) activated an...

Cell cycle dependent expression of volume-activated chloride currents in nasopharyngeal carcinoma cells (CNE-2Z)

Cell cycle-dependent expression of volume-activated chloride currents in nasopharyngeal carcinoma cells.

Patch-clamping and cell image analysis techniques were used to study the expression of the volume-activated Cl(-) current, I(Cl(vol)), and regulatory volume decrease (RVD) capacity in the cell cycle in nasopharyngeal carcinoma cells (CNE-2Z). Hypotonic challenge caused CNE-2Z cells to swell and activated a Cl(-) current with a linear conductance, negligible time-dependent inactivation, and a re...

Whole Blood Volume Determined by Radiochromium - Tagged Red Cells

IN CONGESTIVE heart failure the blood volume has generally been considered to be elevated." 6, 14, 15, 16, 18, 21t 24 Recent investigations by Prentice and co-workers19 and by Ross and associates,20 using P32-tagged erythrocytes, have suggested that the blood volume in heart failure often is not increased. The importance of hypervolemia in both the physiology and therapy of cardiac failure has ...

Volume-activated chloride currents from human fibroblasts: blockade by nimodipine.

The whole-cell patch clamp technique was used to identify and to characterize volume-activated Cl- current (ICl(vol)) in fibroblasts derived from human periodontal ligament. During osmotic cell swelling, the cells exhibited an outwardly rectifying current, which was dependent upon the concentration of external Cl-. The anion permeability sequence of the chloride channel for anions was as follow...