Volatile Profiling Aided in the Isolation of Anti-Proliferative Lupeol from the Roots of Clinacanthus nutans (Burm. f.) Lindau

Isolation of anti-proliferative compounds from plants is always hindered by the complexities plant’s nature and tedious processes. Clinacanthus nutans (Burm. f.) Lindau a medicinal plant with reported activities. Our study aimed to isolate potential present in C. plant. To start with, for our study, we came up strategy first profiling volatile leaf, stem root using GC-MS. Comparing profiles greatly narrowed down target study. We decided isolation characterization pentacyclic terpenoid, i.e., lupeol roots nutans, as this compound was found abundantly compared leaf or stem. developed simple maceration re-crystallization method, without necessity go through fractionation column chromatography lupeol. Characterizations isolated identified The activity further investigated against MCF-7 cell line, which showed comparable authentic camptothecin. profile every part first, followed selection most suitable targeted proved useful bioactive nutans.