Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue
نویسندگان
چکیده
منابع مشابه
Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue.
BACKGROUND Controlled-rate freezing of ovarian cortical tissue for preservation of fertility among young women facing chemo- or radio-therapy is a widely accepted procedure. To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus slow programmed freezing. METHODS Ovarian tissue from 20 women, donated...
متن کاملHuman ovarian tissue: vitrification versus conventional freezing.
Sir, In the challenging paper by Wang et al. (2008), the authors have reported about an effective method of vitrification of human ovarian tissue with direct contact of cells in liquid nitrogen, named needle immersed vitrification. Childbirth after cryopreservation of ovarian tissue is now a reality (Donnez et al., 2004; Demeestere et al., 2007; Meirow et al., 2007; Andersen et al., 2008) and t...
متن کاملI-10: Vitrification of Baboon Ovarian Tissue:Will Vitrification Replace Conventional Freezing for Ovarian Tissue Cryopreservation?
Background: The aim of this study was to evaluate the efficacy of a vitrification protocol developed by our group to cryopreserve ovarian tissue. For this, we used ovaries from non-human primates in order to have an animal model close to the clinical setting. Materials and Methods: Ovarian biopsies from five adult baboons were vitrified, warmed and autografted. After five months, follicle survi...
متن کاملProspective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification.
OBJECTIVE To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. DESIGN Prospective randomized. SETTING Academically affiliated, private fertility center. PATIENT(S) Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were ran...
متن کاملVitrification versus conventional cryopreservation technique
During in vitro fertilization treatment (IVF), a surplus of oocytes is produced which results in a surplus of embryos which must be preserved. Due to the recent tendency in ART treatment to transfer fewer embryos in order to avoid multiple pregnancies, partly a result of new laws and guidelines aimed at controlling the number of embryos to be transferred and the number of oocytes to be fertiliz...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2009
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/dep079