Vitrification of canine cumulus-oocyte complexes in DAP213 with a cryotop holder
نویسندگان
چکیده
منابع مشابه
Morphology of canine cumulus-oocyte complexes in pre-pubertal bitches.
The morphology of canine cumulus-oocyte complexes (COCs) before puberty is still unknown. Therefore, the aim of our study was to elucidate the morphological characteristics of pre-pubertal oocytes and cumulus cells by light microscopy, scanning electron microscopy and transmission electron microscopy. The pre-pubertal oocyte was characterized by accumulation of lipid yolk droplets in the cytopl...
متن کاملCryobiological effects of cryoprotectants on morphology of cumulus oocyte complexes (COCs) of sheep using vitrification
The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocyte using vitrification. The vitrification of sheep oocyte was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more e...
متن کاملCryobiological effects of cryoprotectants on morphology of cumulus oocyte complexes (COCs) of sheep oocytes using vitrification
The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocytes using vitrification. The vitrification of sheep oocytes was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more...
متن کاملP-110: Effect of Increasing Amount of Oocyte Secreted Factors on Cumulus Expansion of Bovine Cumulus-Oocyte Complexes
Background: In vitro maturation is a good method to decrease cancer risk of superovulation by gonadotropin hormones. A paracrine effect of oocyte secretions on oocyte developmental competence is under investigation. Apart from oocyte maturation, ovulation in vivo requires a precise control of extracellular matrix modification. Cumulus cells secrete hyaluronan to form a muco-elastic extracellula...
متن کاملThe effect of vitrification on mouse oocyte apoptosis by cryotop method.
BACKGROUND Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. METHOD A total of 200 germinal vesicle (...
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ژورنال
عنوان ژورنال: Reproductive Medicine and Biology
سال: 2010
ISSN: 1445-5781
DOI: 10.1007/s12522-010-0045-6