Vitamin B12 and Deoxyribose Synthesis in Lactobacillus leichmannii

Survival of Lactobacillus Leichmannii in Relation to Vitamin B12 Assays.

Since washed cells of Lactobacillus leichmannii ATCC 7830 frequently showed erratic results in vitamin B(12) assays, a depletion technique was used to stabilize the inoculum. The method consisted of incubating the twice-washed cells at 37 C for 45 min in distilled water. Survival and growth studies indicated that the stabilizing procedure (i) did not affect cell numbers, (ii) was optimal for de...

The microbiological assay of vitamin B12 with Lactobacillus leichmannii.

Purine and pyrimidine requirements of Lactobacillus leichmannii in the presence of vitamin B12 or thymidine.

The role of vitamin B12 in thymine biosynthesis by Lactobacillus leichmannii.

The biochemistry of vitamin Biz has been the subject of several recent reviews (1, 2, 3). Evidence currently available indicates that the vitamin is required for the reduction of formate to methionine and choline methyl. Indirect evidence implicates vitamin Biz in thymine biosynthesis. Thymine will replace the Blz requirements in certain microorganisms (3) and will induce a reticulocyte respons...

Biosynthesis of thymine and formyltetrahydrofolate by vitamin B12-deficient cells of Lactobacillus leichmannii.

A relationship between the functions of vitamin Blz and folic acid (pteroylglutamic acid) is implied by the hematological response induced by either of these vitamins in patients with pernicious anemia (1,2). This interrelationship between the two vitamins may arise because of separate functions at different steps in the formation of common products, such as thymidine, or because vitamin Bit ha...