Villin Family Members Associated with Multiple Stress Responses in Cotton

Villin (VLN) is considered to be one of the most important actin-binding proteins, participates in modulating actin cytoskeleton dynamics, plays essential role plant development and resisting adverse environments. However, systematic studies VLN gene family have not been reported cotton (Gossypium). In this study, 14 GhVLNs were identified G. hirsutum. These GhVLN genes distributed 6 A-subgenome chromosomes D-subgenome allotetraploid upland classified into three phylogenetical groups based on classification model AtVLNs. addition, highly conserved structure motif architecture. The number introns was ranged from 18 22 length protein sequences varied 901 1077. Six gelsolin homology domains, G1–G6, villin headpiece domain, VHP, existed all with exception two VLNs (GhVLN6 GhVLN13) which lacked VHP. Cis-elements analysis revealed that promoter regions contained various light related components also elements responsible for phytohormones stresses response, indicating that, when subjected those environments, plants may activate response system by targeting survive crisis. Heatmaps showed exhibited expression patterns, some accumulated certain tissues, root, petal, stamen or elongating fibers, obviously induced environmental changes. Especially GhVLN3 GhVLN10 preferentially expressed fibers distinctly upregulated abiotic (salt, PEG, cold heat) biotic (Verticillium dahliae V991) stresses. This study provide useful information biological function identification resources creating high-quality resistant germplasms.