منابع مشابه
Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factor is a tetramer of ...
متن کاملVesicular Transport: EnSNAREd by GAPs
How are SNARE proteins included into transport vesicles? One way is through the interaction with ArfGAP proteins. A recent study reports that the ArfGAP Hrb can wrap around the SNARE VAMP7, causing its endocytosis by clathrin-coated vesicles.
متن کاملVesicular transport earns a Nobel.
The field of intracellular traffic is celebrating the awarding of the 2013 Nobel Prize in Physiology or Medicine to three of its most distinguished scientists--James Rothman, Randy Schekman, and Thomas Südhof--for their discoveries on the molecular mechanisms of vesicular transport. Their outstanding achievements serve as inspiration for the next generation of scientists to continue the task of...
متن کاملPresynaptic HCN Channels Regulate Vesicular Glutamate Transport
The amount of neurotransmitter stored in synaptic vesicles determines postsynaptic quantal size and thus the strength of synaptic transmission. However, little is known about regulation of vesicular neurotransmitter uptake. In recordings from the calyx of Held, a giant mammalian glutamatergic synapse, we found that changes in presynaptic Na(+) concentration above and below a resting value of 13...
متن کاملFunctional Studies of Vesicular Transport in Yeast
Barmark, G. 2005. Functional studies of vesicular transport in yeast. Doctor’s dissertation. ISSN 1652-6880, ISBN 91-576-6909-0 A basic feature of eukaryotic cells is their compartmental organisation. To maintain this cellular order, a strictly regulated intracellular transport is essential. The compartments are defined by their lipid membranes and the transport between different compartments i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Pharmacogenomics Journal
سال: 2001
ISSN: 1470-269X,1473-1150
DOI: 10.1038/sj.tpj.6500055