Versatile transgenic multistage effector-gene combinations for Plasmodium falciparum suppression in Anopheles

Engineered Resistance to Plasmodium falciparum Development in Transgenic Anopheles stephensi

Transposon-mediated transformation was used to produce Anopheles stephensi that express single-chain antibodies (scFvs) designed to target the human malaria parasite, Plasmodium falciparum. The scFvs, m1C3, m4B7, and m2A10, are derived from mouse monoclonal antibodies that inhibit either ookinete invasion of the midgut or sporozoite invasion of salivary glands. The scFvs that target the parasit...

Transgenic Anopheles stephensi coexpressing single-chain antibodies resist Plasmodium falciparum development.

Anopheles stephensi mosquitoes expressing m1C3, m4B7, or m2A10 single-chain antibodies (scFvs) have significantly lower levels of infection compared to controls when challenged with Plasmodium falciparum, a human malaria pathogen. These scFvs are derived from antibodies specific to a parasite chitinase, the 25 kDa protein and the circumsporozoite protein, respectively. Transgenes comprising m2A...

infection rates for some spp . Plasmodium falciparum Anopheles

Presence of circumsporozoite protein (CSP) was Plasmodium falciparum detected by enzyme linked immunosorbent assay (ELISA) in a sample of s.s., and collected in Anopheles gambiae A. melas A. pharoensis Guinea-Bissau during October and November 2009. The percentage of P. infected samples (10.2% overall) was comparable to earlier studies falciparum from other sites in Guinea-Bissau (9.6-12.4%). T...

Human chitotriosidase helps Plasmodium falciparum in the Anopheles midgut.

Epigenetic regulation of Plasmodium falciparum clonally variant gene expression during development in Anopheles gambiae

P. falciparum phenotypic plasticity is linked to the variant expression of clonal multigene families such as the var genes. We have examined changes in transcription and histone modifications that occur during sporogonic development of P. falciparum in the mosquito host. All var genes are silenced or transcribed at low levels in blood stages (gametocyte/ring) of the parasite in the human host. ...