Vegetative propagation of freesia through callus cultures.

Rapid Clonal Propagation of Chrysanthemum through Embroyogenic Callus Formation

Chrysanthemum nodal explants were cultured on 1⁄2 X MS medium containing different combinations of growth hormones. A reasonable callus formed on explants when MS contained 0.5 mg. cm each of BAP and NAA after about 3 weeks. Similar results were obtained on MS + 0.5 mg. cm BAP + 0.1 mg. cm NAA or 1.0 mg. cm BAP + 0.1 mg. cm NAA. A maroonish embryogenic callus was formed on MS containing BAP onl...

Sugarcane Clones Derived through Immature Leaf Callus Cultures

The biotechnological potential of pectinolytic fungi isolated from citrus fruits was studied. Seventy five fungal isolates were isolated from decayed citrus fruits. All of these isolates were screened for their pectinolytic activity. Thirty six out of seventy five isolates proved to have pectinolytic activity. These isolates were identified and classified into twenty strains belonging to eleven...

Cell-to-cell transport through plasmodesmata in tree callus cultures.

One factor that contributes to a successful fruit tree grafting is the establishment of symplasmic contacts in the graft interface to facilitate the transfer of compounds between scion and stock. Using novel experimental and theoretical approaches we investigated whether the localized incompatibility, experienced in some Prunus grafts, could be related to insufficient plasmodesmal coupling at a...

Callus and Differentiated Soybean Root Cultures

The metabolism of 11-14C12,4-dichlorophenoxyacetic acid (2,4-D) in soybean ( Glycine max IL.1 Merrill var. Amsoy) root callus and in differentiated soybean root cultures was investigated as a function of pesticide concentration and age of tissue. The chronological age of the tissue was found to be correlated with the mitotic index which reached a peak at 2 weeks and then declined. The metabolis...

Propagation of goat pox virus on monolayer cell cultures