Varying contributions of three ryanodine receptor point mutations to diamide insecticide resistance in <i>Plutella xylostella</i>

BACKGROUND Although decoding the molecular mechanisms underlying insecticide resistance has often proven difficult, recent progress revealed that specific mutations in ryanodine receptor (RyR) of diamondback moth, Plutella xylostella, can confer to diamide insecticides. The extent which RyR contribute phenotype, associated genetic traits and fitness costs remain limited. RESULTS Three field-evolved PxRyR (G4946E, I4790 M, K) were respectively introgressed into a common susceptible background strain (IPP-S) P. xylostella with marker-assisted backcrossing. alone result moderate high levels five commercial diamides (flubendiamide, chlorantraniliprole, cyantraniliprole, tetraniliprole, cyclaniliprole), intensity mediated by three was hierarchical order K (1199- >2778-fold) > G4946E (39- 739-fold) M (16- 57-fold). Flubendiamide autosomal incompletely recessive, significantly linked constructed strains. In addition, flubendiamide hybrid progeny from any two resistant strains fell between status their parents. Furthermore, comparing net replacement rate, 4946E, 4790 0.77, 0.93 0.92 relative IPP-S strain, respectively. CONCLUSION independent varying degrees xylostella. Among mutations, confers highest (> 1000-fold) all diamides. findings guide management practices for other arthropods.