Variations in Human Serum Lipoprotein Detected by Isoelectric Focusing
نویسندگان
چکیده
منابع مشابه
A cortisol-induced charge difference in desialylated human transcortin detected by isoelectric focusing.
Human desialylated transcortin was found to be microheterogeneous by isoelectric focusing. All samples tested showed two cortisol-binding components. Liganded with cortisol these components are isoelectric at pH 5.2 and 5.4. In the unliganded form, however, they eluted at pH 5.4 and 5.6, respectively. This cortisol-controlled transition was reversible in both directions. Isolated components con...
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1. Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of hum...
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Analytical isoelectric focusing (IEF) has been applied to the study of the apolipoprotein components of rat serum high density and very low density lipoproteins. The apolipoproteins were separated on 7.5% polyacrylamide gels containing 6.8% urea, with a pH gradient of 4-6. The middle molecular weight range apolipoproteins were identified on IEF gels by the use of apolipoproteins purified by ele...
متن کاملDetection of recombinant human erythropoietin in urine by isoelectric focusing.
BACKGROUND Doping with erythropoietic proteins such as recombinant human erythropoietin (rHuEPO) and darbepoetin alfa is a serious issue in sport. There is little information on the time course of detection of rHuEPO in urine and on methods to evaluate electrophoresis-based data. METHODS We used a recently described isoelectric focusing method for detecting rHuEPO and endogenous EPO in urine ...
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Background To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1971
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1971.9.6.503