Variation of egg proteins between bird varieties by using SDS-PAGE

Identification of electrophoretic proteins pattern of Fusaium verticillioides isolates from maize by SDS-PAGE.

Study on pattern of Neospora caninumtachyzoite proteins by SDS-PAGE and Western blotting in aborted cows

The intracellular parasite Neospora caninumis prevalent in several countries and is increasingly recognized as an important cause of abortion and stillbirth in cattle. For characterizeing the tachyzoite antigens of Neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the third semester of the pregnancy period and had antibodies to Neospora caninumin ELISA. To ...

Analysis of interactions between carbohydrates and proteins using fluorescent labeling and SDS-PAGE.

A rapid method for the analysis of carbohydrate-protein interactions by using fluorescent labeling and SDS-PAGE was developed. The N-acetyl-beta-D-glucosamine-WGA complex and alpha-D-mannose-Con A complex were labeled with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS). The protein band displaying fluorescence with ultraviolet illumination was seen after SDS-PAGE.

Proteomic study of muscle sarcoplasmic proteins using AUT-PAGE/SDS-PAGE as two-dimensional gel electrophoresis.

In the present study, an alternative procedure for two-dimensional (2D) electrophoretic analysis in proteomic investigation of the most represented basic muscle water-soluble proteins is suggested. Our method consists of Acetic acid-Urea-Triton polyacrylamide gel (AUT-PAGE) analysis in the first dimension and standard sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) in the second dimension...

Crude Protein Profiling of Varieties of Capsicum annuum and Capsicum frutescens using SDS-PAGE

The study was aimed at assessing the genetic variation and relatedness among four Capsicum varieties through electrophoretic separation of their leaf and seed proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total seed and leaf proteins were extracted and separated on 12% polyacrylamide gels using standard protocols. Distinct polymorphism in electrophoretic ban...