Validity of western immunoblot band patterns in the serodiagnosis of Lyme borreliosis
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چکیده
منابع مشابه
Serodiagnosis of Lyme borreliosis by western immunoblot: reactivity of various significant antibodies against Borrelia burgdorferi.
The significance of various antibodies against Borrelia burgdorferi was studied by Western blot (immunoblot) by using 578 human serum samples. The proteins regularly detected by using samples from patients with Lyme borreliosis were those with bands with molecular masses of 94, 83, 75, 66, 60, 55, 46, 41, 39, 34, 31, 29, 22, and 17 kDa. The detectable frequencies of most of these proteins appea...
متن کاملValidity of interpretation criteria for standardized Western blots (immunoblots) for serodiagnosis of Lyme borreliosis based on sera collected throughout Europe.
Western blotting (WB; immunoblotting) is a widely used tool for the serodiagnosis of Lyme borreliosis (LB), but so far, no generally accepted criteria for performance and interpretation have been established in Europe. The current study was preceeded by a detailed analysis of WB with whole-cell lysates of three species of Borrelia burgdorferi sensu lato (U. Hauser, G. Lehnert, R. Lobentanzer, a...
متن کاملTHE FIRST ENDEMIC CASE OF LYME BORRELIOSIS IN IRAN
This case report shows the existence of Lyme borreliosis disease in Iran and proves the existence of the spirochete Borrelia burgdorferi in Iran which had not been found before in the ticks of this region. It is important for our physicians to consider Lyme borreliosis in their differential diagnosis. Apart from skin manifestations, neurological, cardiac, articular and ocular lesions are no...
متن کاملImmunoblot interpretation criteria for serodiagnosis of early Lyme disease.
We monitored the antibody responses of 55 treated patients with early Lyme disease and physician-documented erythema migrans. Six sequential serum samples were obtained from patients before, during, and until one year after antibiotic therapy and analyzed by in-house enzyme-linked immunosorbent (ELISA) and immunoblot assays. An immunoblot procedure utilizing a gradient gel and an image analysis...
متن کاملThe Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early lyme disease is the flaA gene product.
The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to the P37 antigen. DNA sequence analysis of this clone revealed the identity of ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1991
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.29.1.174-182.1991