Validation of reference genes in human chordoma
نویسندگان
چکیده
منابع مشابه
Validation of reference genes in human chordoma
BACKGROUND Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference genes. Using an unstable reference...
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Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...
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ژورنال
عنوان ژورنال: Surgical Neurology International
سال: 2017
ISSN: 2152-7806
DOI: 10.4103/sni.sni_399_16