Validation of Diagnostic Markers for Streak Virus Disease Resistance in Maize
نویسندگان
چکیده
Maize streak virus (MSV) is responsible for disease of maize and poses a serious threat to production in sub-Saharan Africa. Polygenic resistance MSV has become an essential requirement modern cultivars mitigate yield losses. Many single nucleotide polymorphism (SNP) markers linked putative loci have been identified use forward breeding. This study aimed validate, using the high-throughput kompetitive allele specific PCR (KASP) assay, diagnostic ability three SNP Msv1 trait 151 early generations inbred lines with diverse genetic backgrounds, together nine MSV-resistant elite susceptible check (cv. Pool-16). The phenotypic responses were determined by inoculation viruliferous leafhoppers (Cicadulina triangular) under screenhouse conditions. Based on established rating system, categorized into resistant, moderately susceptible, highly susceptible. SNPs associated detected 133 lines, which as resistant (54), (76), (1). 18 generation without these classified (10), (5), (3). confirms strong association their usefulness breeding while emphasizing need identify additional screen any ambiguity.
منابع مشابه
Inducible Resistance to Maize Streak Virus
Maize streak virus (MSV), which causes maize streak disease (MSD), is the major viral pathogenic constraint on maize production in Africa. Type member of the Mastrevirus genus in the family Geminiviridae, MSV has a 2.7 kb, single-stranded circular DNA genome encoding a coat protein, movement protein, and the two replication-associated proteins Rep and RepA. While we have previously developed MS...
متن کاملReconstructing the history of Maize streak virus
64 65 Maize streak virus (MSV) strain A, the causal agent of Maize streak disease is 66 today one of the most serious biotic threats to African food security. Determining 67 where MSV-A originated and how it spread trans-continentally could yield 68 valuable insights into its historical emergence as a crop pathogen. Similarly, 69 determining where the major extant MSV-A lineages arose could ide...
متن کاملIDENTIFICATION OF MOLECULAR MARKERS LINKED TO LEAF CURL VIRUS DISEASE RESISTANCE IN COTTON
The identification of molecular markers linked to leaf curl virus (CLCuV) disease resistance in cotton has the potential to improve both the efficiency and the efficacy of selection in cotton breeding programs. Genetic analysis suggested that CLCuV resistance is controlled by a single dominant gene. In this study, an interspecific F2 population derived from a cross of Gossypium barbadense and G...
متن کاملThe Effect of High Temperature Treatment on Wheat Streak Mosaic Virus Resistance and Certain Resistance-Related Chemicals in Bread Wheat
Background and Aims: To evaluate the effect of temperature on wheat streak mosaic virus (WSMV) resistance phenotype, through total protein, phenol, and peroxidase activity in bread wheat, a factorial experiment was conducted using Adl-Cross (resistant) and Marvdasht (susceptible) cultivars. Materials and Methods: Results showed that incubation at 32C changed the gene expression for resistance ...
متن کاملStructure of the Maize streak virus geminate particle.
The Geminiviridae is an extensive family of plant viruses responsible for economically devastating diseases in crops worldwide. Geminiviruses package circular, single-stranded DNA (ssDNA) genomes. The characteristic twinned or "geminate" particles, which consist of two joined, incomplete T = 1 icosahedra, are unique among viruses. We have determined the first structure of a geminivirus particle...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agriculture
سال: 2021
ISSN: ['2077-0472']
DOI: https://doi.org/10.3390/agriculture11020130