Validation of Anthrax specific pagA quantitative PCR for detection of Bacillus anthracis pXO1 plasmid
نویسندگان
چکیده
منابع مشابه
Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility
The large plasmid pXO1 encoding the anthrax toxin is important for the virulence of Bacillus anthracis. It is essential to cure pXO1 from B. anthracis to evaluate its role in the pathogenesis of anthrax infection. Because conventional methods for curing plasmids (e.g., curing agents or growth at elevated temperatures) can induce mutations in the host chromosomal DNA, we developed a specific and...
متن کاملSequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes.
The Bacillus anthracis Sterne plasmid pXO1 was sequenced by random, "shotgun" cloning. A circular sequence of 181,654 bp was generated. One hundred forty-three open reading frames (ORFs) were predicted using GeneMark and GeneMark.hmm, comprising only 61% (110,817 bp) of the pXO1 DNA sequence. The overall guanine-plus-cytosine content of the plasmid is 32.5%. The most recognizable feature of the...
متن کاملRap phosphatase of virulence plasmid pXO1 inhibits Bacillus anthracis sporulation.
This study shows that the Bacillus anthracis pXO1 virulence plasmid carries a Rap-Phr system, BXA0205, which regulates sporulation initiation in this organism. The BXA0205Rap protein was shown to dephosphorylate the Spo0F response regulator intermediate of the phosphorelay signal transduction system that regulates the initiation of the developmental pathway in response to environmental, metabol...
متن کاملA new minimal replicon of Bacillus anthracis plasmid pXO1.
An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5.95-kb region encompassing three putative genes was identified as the minimal p...
متن کاملBacillus anthracis pXO1 plasmid encodes a putative membrane-bound bacteriocin
Evolutionary advantages over cousin cells in bacterial pathogens may decide about the success of a specific cell in its environment. Bacteria use a plethora of methods to defend against other cells and many devices to attack their opponents when competing for resources. Bacteriocins are antibacterial proteins that are used to eliminate competition. We report the discovery of a putative membrane...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal for Veterinary Medicine, Biotechnology and Biosafety
سال: 2019
ISSN: 2411-0388,2411-3174
DOI: 10.36016/jvmbbs-2019-5-2-3