Validation of a lipoprotein(a) particle concentration assay by quantitative lipoprotein immunofixation electrophoresis
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چکیده
منابع مشابه
Serum protein electrophoresis and immunofixation by a semiautomated electrophoresis system.
Semiautomated agarose electrophoresis and immunofixation performed with Hydrasys-Hyrys (Sebia) were compared with conventional, manually performed methods, including cellulose acetate electrophoresis, immunoelectrophoresis, and immunofixation. Reference intervals for agarose electrophoresis with Hydrasys-Hyrys were determined. Within-run imprecision (CV) for fraction quantitation with the semia...
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The electrophoretic mobilities of low density lipoprotein (LDL) and six pure proteins in a 0.5% agarose gel have been compared to literature electrophoretic mobility values determined by the Tiselius moving boundary method. There is a strong correlation (r = 0.99) between the electrophoretic mobilities determined by the two techniques. The electrophoretic behavior of charged particles smaller t...
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addressed some recently recognized problems in testing concentrated urine for monoclonal free light chains of immunoglobulins, i.e., Bence Jones protein (BJP), by high-resolution elec-trophoresis (lIRE) and immunofix-ation electrophoresis (IFE). When BJP is present in low concentrations, poly-clonal free light chains can interfere with identification of the protein either by causing a dense bac...
متن کاملSensitivity of immunofixation electrophoresis for detecting IgM paraproteins in serum.
Detection and characterization of small IgM paraproteins by immunoelectrophoresis are often difficult because of (a) slow diffusion of the macromolecular immunoglobulin, and (b) the obscuring of the light chain, especially kappa, by the light chains of background polyclonal immunoglobulin (the "umbrella effect"). Various methods of sample pretreatment have been tried in an attempt to overcome t...
متن کاملImmunofixation. I. General principles and application to agarose gel electrophoresis.
Immunofixation offers the worker an economical means of physically locating a protein in an electrophoretic strip and is ideally suited to forensic medicine, genetic studies, or research. The method is as simple and economical as the commonly used one- or two-dimensional immunoelectrophoresis, yet yields considerably more information.
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ژورنال
عنوان ژورنال: Clinica Chimica Acta
سال: 2015
ISSN: 0009-8981
DOI: 10.1016/j.cca.2014.10.013