Validation of a blood group genotyping method based on high-resolution melting curve analysis

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The LightTyper: high-throughput genotyping using fluorescent melting curve analysis.

Instrumentation, chemistry, and software for high-throughput genotyping using fluorescent melting curves are described. The LightTyper system provides post-amplification genotyping within 10 min using samples in 96- or 384-well microplate formats. The system is homogenous because all reagents are added at the beginning of the reaction and there is no sample manipulation between amplification an...

متن کامل

Genotyping of OATP2 Variants in a Group of Malaysian Neonates Using High-Resolution Melting Analysis

Organic anion transport polypeptide 2 (OATP2), also known as OATP-C, OATP1B1, and LST-1, is responsible for the transportation of organic anions into hepatocytes. It has been reported that OATP2 transports a broad range of endogenous and xenobiotic compounds such as bile acids, bilirubin, sulfate and glucuronide conjugates, thyroid hormones, peptides, and drugs like 3-hydroxyl-3 methylglutarylc...

متن کامل

Development of a High-Resolution Melting Analysis Method for CYP2C19*17 Genotyping in Healthy Volunteers

BACKGROUND Genetic polymorphisms of drug metabolisms by cytochrome P450 (P450s) could affect drug response, attracting particular interest in the pharmacogenetics. Due to the importance of CYP2C19* 17 allele and its capability of super- fast metabolism and also lack of information about distribution of the alleles in Iranian population, this research aimed to use High Resolution Melting (HRM) m...

متن کامل

development of a high-resolution melting analysis method based on sybr green-i for rs7216389 locus genotyping in asthmatic child patients

background: asthma is caused by the combination of different factors. current concepts of asthma pathogenesis emphasize on gene-environment interactions. mega-genome scanning projects revealed that different single nucleotide polymorphisms (snps) are related to asthma susceptibility. rs7216389-t is one of them that is related to childhood asthma and its effect on childhood asthma severity has b...

متن کامل

Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device.

We developed a thermal digital microfluidic (T-DMF) device enabling ultrafast DNA melting curve analysis (MCA). Within 7 seconds, the T-DMF device succeeded in differentiating a melting point difference down to 1.6 °C with a variation of 0.3 °C in a tiny droplet sample (1.2 μL), which was 300 times faster and with 20 times less sample spending than the standard MCA (35 minutes, 25 μL) run in a ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Immunohematology

سال: 2019

ISSN: 0894-203X,1930-3955

DOI: 10.21307/immunohematology-2019-114