VALIDATION AND APPLICATION OF RP-HPLC METHOD FOR QUANTIFICATION OF ENROFLOXACIN IN PURE AND VETERINARY DOSAGE FORMS

Objective: The main objective of this study is to develop and validate a simple, new, fast, sensitive, precise accurate RP-HPLC analytical methods have been established for the estimation enrofloxacin in bulk pharmaceutical dosage forms.Methods: present method was developed validated on Waters HPLC system using Phenomenex make Shimadzu C18 column (250 mm × 4.6 i.d., 5μm particle size) used separation. Best results were obtained with mobile phase composition consisting Acetonitrile-water (80:20, v/v). regulated at 1.0 ml/min flow rate 270 nm UV detection. Results: Enrofloxacin eluted 3.405 min retention time. parameters such as accuracy, precision, linearity, LOD, LOQ, ruggedness, robustness validating according ICH guidelines. Linearity exhibited over concentration range 0.1-0.6µg/ml Limit Detection Quantitation values 0.001µg/ml 0.03µg/ml, respectively. result analysis shows that amount drugs formulation has very good correlation label claim %RSD will be less than 2 all validation parameters. Recoveries studies revealed within specified limits.Conclusion: various per Hence proposed found satisfactory could routine their marketed formulation.