Validating Antibodies to the Cannabinoid CB2 Receptor

Validating Antibodies to the Cannabinoid CB2 Receptor

The problem of antibody reliability in research has now reached the point where many journals reject any work that lacks adequate controls for antibody validation. Despite this, it is still common for researchers to rely on positive controls and only minimal negative controls (such as blocking peptide controls). Using careful cross-comparisons between antibody labeling and other labeling techni...

Peripheral cannabinoid receptor, CB2, regulates bone mass.

The endogenous cannabinoids bind to and activate two G protein-coupled receptors, the predominantly central cannabinoid receptor type 1 (CB1) and peripheral cannabinoid receptor type 2 (CB2). Whereas CB1 mediates the cannabinoid psychotropic, analgesic, and orectic effects, CB2 has been implicated recently in the regulation of liver fibrosis and atherosclerosis. Here we show that CB2-deficient ...

Cannabinoid Receptor CB2 Modulates Axon Guidance

Navigation of retinal projections towards their targets is regulated by guidance molecules and growth cone transduction mechanisms. Here, we present in vitro and in vivo evidences that the cannabinoid receptor 2 (CB2R) is expressed along the retino-thalamic pathway and exerts a modulatory action on axon guidance. These effects are specific to CB2R since no changes were observed in mice where th...

Cannabinoid Inhibition of Macrophage Migration to the Tat Protein of Hiv-1 Is Linked to the Cb2 Cannabinoid Receptor

CB2 Cannabinoid Receptor As Potential Target against Alzheimer's Disease

The CB2 receptor is one of the components of the endogenous cannabinoid system, a complex network of signaling molecules and receptors involved in the homeostatic control of several physiological functions. Accumulated evidence suggests a role for CB2 receptors in Alzheimer's disease (AD) and indicates their potential as a therapeutic target against this neurodegenerative disease. Levels of CB2...